NlaIII from New England Biolabs

Product Specs
ItemNlaIII
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0125S
Concentration10 u/µL
Quantity500 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-80 C

Sequence

CATG/

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in ArabidopsisNature CommunicationsNovember 27, 2023Qin Li, Jincong Zhou, Shuai Li, Weifeng Zhang, Yingxue Du, Kuan Li, Yingxiang Wang, Qianwen Sunreferences34,35. In brief, 1 μg fragmented DNA (digested by DdeI (R0175S, NEB), MseI (R0525S, NEB), NlaIII (R0125S, NEB) and MboI (R0147S, NEB) endonucleases) was used for S9.6 immunoprecipitation. Meanwhile, the same

(2) DeSUMOylation of chromatin-bound proteins limits the rapid transcriptional reprogramming induced by daunorubicin in acute myeloid leukemiasNucleic Acids ResearchSeptember 8, 2023Mathias Boulanger, Mays Aqrouq, Denis Tempé, Chamseddine Kifagi, Marko Ristic, Dana Akl, Rawan Hallal, Aude Carusi, Ludovic Gabellier, Marion de Toledo, et al.pellets were resuspended in 700 μl of first enzyme manufacturer buffer 1X (NlaIII – cutsmart [NEB – R0125L]) and homogenized on ice (50 strokes in total) with a 1 ml Dounce homogenizer. Cells were permeabilized

(3) Differentiation block in acute myeloid leukemia regulated by intronic sequences of FTOiScienceAugust 18, 2023Francesco Camera, Isabel Romero-Camarero, Bradley H. Revell, Fabio M.R. Amaral, Oliver J. Sinclair, Fabrizio Simeoni, Daniel H. Wiseman, Lovorka Stojic, Tim C.P. SomervailleTechnologies,Cat#1081059 XbaI,NEB,Cat#R0145L SbfI,NEB,Cat#R3642L Age1,NEB,Cat#R3552S EcoR1-HF,NEB,Cat#R3101L DpnII,NEB,Cat#R0543S NlaIII,NEB,Cat#R0125S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, RNeasy Plus

(4) Copy number variation alters local and global mutational toleranceGenome ResearchAugust 1, 2023Grace Avecilla, Pieter Spealman, Julia Matthews, Elodie Caudal, Joseph Schacherer, David Greshamsample, 2 μg of DNA was digested using DpnII (NEB R0543L), and 2 μg of DNA was digested with NlaIII (NEB R0125L). The reactions were heat inactivated, circularized by ligation using T4 Ligase (Thermo Fisher Scientific

(5) Multiomic analysis of cohesin reveals that ZBTB transcription factors contribute to chromatin interactionsNucleic Acids ResearchJuly 21, 2023Rui Wang, Qiqin Xu, Chenlu Wang, Kai Tian, Hui Wang, Xiong Jitemperature. The nuclei were isolated and treated with 25 μl of 10× cutsmart buffer and 100 U of NlaIII (NEB, #R0125S), and incubated overnight at 37°C, 900 rpm to digest the chromatin. The next day, the restriction enzyme

(6) Contribution of the IGCR1 regulatory element and the 3′ Igh CTCF-binding elements to regulation of Igh V(D)J recombinationProceedings of the National Academy of SciencesJune 27, 2023Zhuoyi Liang, Lijuan Zhao, Adam Yongxin Ye, Sherry G. Lin, Yiwen Zhang, Chunguang Guo, Hai-Qiang Dai, Zhaoqing Ba, Frederick W. Altand protease inhibitors (Roche, #11836153001). Nuclei were digested with 700 units of NlaIII (NEB, #R0125) restriction enzyme at 37°C overnight, followed by ligation (T4 DNA ligase NEB M0202L) at 16°C overnight.

(7) The genome-wide mutational consequences of DNA hypomethylationScientific ReportsApril 27, 2023Nicolle Besselink, Janneke Keijer, Carlo Vermeulen, Sander Boymans, Jeroen de Ridder, Arne van Hoeck, Edwin Cuppen, Ewart Kuijkheat inactivation at 80 °C for 10 min. The genomic DNA was subsequently fragmented with 100 nl NLAIII (R0125L, New England Biolabs) in 1 × Cutsmart (New England Biolabs) for 2 h at 37 °C followed by heat inactivation

(8) Elevated enhancer-oncogene contacts and higher oncogene expression levels by recurrent CTCF inactivating mutations in acute T cell leukemiaCell ReportsApril 14, 2023Willem K. Smits, Carlo Vermeulen, Rico Hagelaar, Shunsuke Kimura, Eric M. Vroegindeweij, Jessica G.C.A.M. Buijs-Gladdines, Ellen van de Geer, Marjon J.A.M. Verstegen, Erik Splinter, Simon V. van Reijmersdal, et al.,Beckman Coulter,Cat# A63880 Formaldehyde solution,Merck,Cat# 252549; CAS: 50–00-0 NlaIII,New England Biolabs,Cat# R0125S T4 DNA ligase,New England Biolabs,Cat# M0202S NspI,New England Biolabs,Cat# R0602S TRIzol Reagent,Thermo Fisher

(9) An autoimmune pleiotropic SNP modulates IRF5 alternative promoter usage through ZBTB3-mediated chromatin loopingNature communicationsMarch 3, 2023Wang Z, Liang Q, Qian X, Hu B, Zheng Z, Wang J, Hu Y, Bao Z, Zhao K, Zhou Y, et al.alcohol (25:24:1) (ThermoFisher, 15593-049). The purified DNA samples were digested with NlaIII (NEB, R0125L) for the rs4728142-containing region (GRCh37/hg19 chr7: 128,573,714–128,574,338) or CviQI (NEB, R0639L)

(10) T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formationeLifeJanuary 31, 2023Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, et al.the Weintraub A.S. et al. protocol (Weintraub et al., 2017). Briefly, NlaIII (New England Biolabs, R0125) was used as the primary cutter and DpnII (New England Biolabs, R0543) as a secondary cutter. Touchdown

(11) Strand asymmetry influences mismatch resolution during a single-strand annealingGenome BiologyDecember 1, 2022Victoria O. Pokusaeva, ArÃ¡nzazu Rosado Diez, Lorena Espinar, Albert TorellÃ³ PÃ©rez, Guillaume J. FilionInverse PCR Ten micrograms of genomic DNA from transfected mESCs were digested using 10 μl 10 U/μL NlaIII (NEB, #R0125S) in a 50 μL final volume for 3 h at 37 °C. The reaction was heat-inactivated at 65 °C for 20 min. The

(12) Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphismsNature CommunicationsDecember 1, 2022Piaopiao Chen, Agnès H. Michel, Jianzhi ZhangGenomic DNA (2 × 2 μg) was separately digested with 50 units of DpnII (NEB #R0543L) and NlaIII (NEB #R0125L). Each of these four-cutter restriction enzymes has one cutting site approximately every 256 nucleotides,

(13) Hi-TrAC reveals division of labor of transcription factors in organizing chromatin loopsNat CommunNovember 5, 2022Shuai Liu, Yaqiang Cao, Kairong Cui, Qingsong Tang, Keji Zhaoelution buffer and digested with the restriction enzymes 2 μL MluCI (NEB, R0538L) and 2 μL NlaIII (NEB, R0125L) in 100 μL reaction mixture at 37 °C for 30 min. The reaction mixture was adjusted to 1x B&W buffer

(14) EWS/FLI mediated reprogramming of 3D chromatin promotes an altered transcriptional state in Ewing sarcomaNucleic Acids ResearchSeptember 23, 2022Iftekhar A Showpnil, Julia Selich-Anderson, Cenny Taslim, Megann A Boone, Jesse C Crow, Emily R Theisen, Stephen L LessnickHutchinson Cancer Center, Seattle, WA, USA), T4 DNA ligase (NEB, M0202), MboI (NEB, R0147), NlaIII (NEB, R0125), Expand Long Template polymerase (Roche, 11759060001), RNAse A (ThermoFisher Scientific, AM2271), Proteinase K

(15) Chromatin organizer SATB1 controls the cell identity of CD4+ CD8+ double-positive thymocytes by regulating the activity of super-enhancersNature CommunicationsSeptember 22, 2022Delong Feng, Yanhong Chen, Ranran Dai, Shasha Bian, Wei Xue, Yongchang Zhu, Zhaoqiang Li, Yiting Yang, Yan Zhang, Jiarui Zhang, et al.DNA were purified by ethanol precipitation. Secondary restriction was performed using NlaIII (NEB, R0125L). Secondary ligation was carried out with T4 DNA Ligase in diluted conditions. Ligated DNA products

(16) Deciphering multi-way interactions in the human genomeNature CommunicationsSeptember 20, 2022Gabrielle A. Dotson, Can Chen, Stephen Lindsly, Anthony Cicalo, Sam Dilworth, Charles Ryan, Sivakumar Jeyarajan, Walter Meixner, Cooper Stansbury, Joshua Pickard, et al.on ice. Permeabilized cells were then digested with a final concentration of 1 U/μL of NlaIII (NEB-R0125L) and brought to volume with nuclease-free water to achieve a final 1X digestion reaction buffer in 450

(17) Loss-of-function mutation survey revealed that genes with background-dependent fitness are rare and functionally related in yeastProceedings of the National Academy of SciencesSeptember 13, 2022Elodie Caudal, Anne Friedrich, Arthur Jallet, Marion Garin, Jing Hou, Joseph Schacherer(Invitrogen). gDNA (2 × 2 µg) was digested in parallel with 50 U DpnII (NEB, R0543L) and NlaIII (NEB, R0125L) in 50 µL for 16 h at 37 °C. The enzymatic reactions were inactivated for 20 min at 65 °C and DNA fragments

(18) The Role of Plasma Interleukin-6 Levels on Atherosclerotic Cardiovascular Disease and Cardiovascular Mortality Risk Scores in Javanese Patients with Chronic Kidney DiseaseJournal of Personalized MedicineJuly 10, 2022Hendri Susilo, Mochammad Thaha, Budi Susetyo Pikir, Mochamad Yusuf Alsagaff, Satriyo Dwi Suryantoro, Citrawati Dyah Kencono Wungu, Nando Reza Pratama, Cennikon Pakpahan, Delvac Oceandyfinal extension at 72 °C–5′. PCR products were then incubated with restriction enzyme NIaIII (Cat. No. R0125S, New England Biolabs, Ipswich, MA, USA) at 37 °C for 4 h [34]. The results of the PCR-RFLP showed 119

(19) An optimized 4C-seq protocol based on cistrome and epigenome data in the mouse RAW264.7 macrophage cell lineSTAR ProtocolsJune 1, 2022Zhiqiang Huang, Cheng Wang, Eckardt Treuter, Rongrong Fanproteins","Chemicals, peptides, and recombinant proteins" ,, DpnII,NEB,Cat# R0543M NlaIII,NEB,Cat# R0125L 1 kb DNA ladder,Thermo Fisher,Cat# 10787018 Agarose,Thermo Fisher,Cat# R0492 AMPure beads,Beckman Coulter

(20) The 3′ Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies’ association with histone locus bodiesNature CommunicationsMay 25, 2022Hidefumi Suzuki, Ryota Abe, Miho Shimada, Tomonori Hirose, Hiroko Hirose, Keisuke Noguchi, Yoko Ike, Nanami Yasui, Kazuki Furugori, Yuki Yamaguchi, et al.HIST2H2AB, which are present on chromosomes 1 and 6, respectively. For 4C-seq analysis, we used NlaIII (R0125S; NEB) and DpnII (R0543S; NEB) as RE1 and RE2, respectively. For the data analysis, pipe4C was used with

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Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
October 01, 2007

NlaIII

NlaIII from New England Biolabs

Sequence

New England Biolabs

Citations (93)

Reviews

Protocols

Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis

NlaIII from New England Biolabs

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